We have a new lab member - Emily Kracht! Emily graduated from UF in Spring 2021 with degrees in Chemistry & Anthropology. During her undergraduate research at the Florida Museum, she used mass spec tools to analyze ceramics from Caribbean regions. We are thrilled for her to apply her MS knowledge and chemistry background to the world of marine metabolites! Welcome!
Two marine metabolomics manuscripts led by UW colleagues Angie Boysen and Katherine Heal were published in mSystems this month! Boysen et al., show diel oscillations in particulate metabolites consistent with day-night photosynthetic and redox cycles. Heal et al., show how changes in phytoplankton taxonomy shape particulate metabolites along a latitudinal gradient in the North Pacific.
Figure from Boysen et al., showing diel oscillations in metabolite abundance as well corresponding bacterial transcripts associated with their metabolism.
Ashley has received a 2021 University Scholars Award. Her research project is titled "Determining Preferential Conditions for Phytoplankton Photosynthetic Cycles" where she'll be looking into the relationship between nitrogen metabolism and photochemistry in marine phytoplankton. Congrats!
Graduating senior Mardeliz Martinez has been accepted to Florida State University's Biology graduate program. Starting in August, she’ll be working in their Ecology and Evolution Group with Dr. Scott Burgess on coral biology. Congrats!
Does this image of the timsTOF fleX mass spec excite you!? Keep reading... we are looking for a Research Technician who has interests/experience in LC-MS metabolomics. The technician will operate instruments such as this one and help carry out projects focused on microbial interactions and metabolism. More details about the position can be found here.
Image of the internal components of the Bruker timsTOF fleX system.
Coesel & collaborators through the Simons Foundation SCOPE-Aloha Program have published a new study in PNAS on light sensing in microbial eukaryotes. Using transcriptomes from microbial cultures and metatranscriptomes from the North Pacific surface ocean, Coesel et al. unveil the diversity of light-sensing elements and photoreceptors used by eukaryotic plankton in the open ocean. Ultimately, these receptors synchronize and stabilize plankton patterns of growth, division, metabolism, and mortality within the dynamic ocean environment.
A figure from Coesel et al., "Diel transcriptional oscillations of light-sensitive regulatory elements in open-ocean eukaryotic plankton communities"
Durham has been awarded a one-year award that will support analysis and integration of molecular and oceanographical datasets collected as part of the SCOPE-Gradients Program (SCOPE: Simons Collaboration on Ocean Processes and Ecology). Efforts are focused on understanding how chemical and physical features in the North Pacific influence microbial diversity and activity.
Image of the research crew on the back deck of the R/V Kilo Moana during the SCOPE-Gradients 3 cruise.
We are excited to announce our embarkment into a new project that focuses on metabolite exchange between moss and symbiotic cyanobacteria. Graduate student Rebecca Key will lead the work as part of her dissertation, combining molecular and chemical 'omics measurements to track C, N, and S cycling between moss and associated microbes. The project is supported by JGI’s Community Science Program and led by PI Stuart McDaniel (UF Biology) along with coPIs Bryndan Durham and Todd Rosenstiel (PSU).
Image of moss colonies on agar plates; c/o Rebecca Key.
Over the past month, we have made a slow and staged return back into the lab. Much of our time has been spent working with colleagues in ICBR to get the new LC-MS system installed and up-and-running through remote instruction and training. Check out the story by UF Research.
Left to right, Dr. Sixue Chen (Professor and Director of ICBR Proteomics Facility), Dr. Jin Koh (ICBR Proteomics & Mass Spectrometry Scientific Director), and Dr. Bryndan Durham after installation of the Evosep One and timsTOF fleX system.
Left to right, Dr. Jin Koh, Dr. Wei Zhu (Chen Lab), and Rebecca Key (Durham Lab) work together during instrument training.
Poster reads: "In this lab, we believe: science is real (microscope image), love is love (rainbow anatomical heart), Black lives matter (brown raised fist), feminism is for everyone (female symbol), microbes are cool (single-celled organisms), immigrants are welcome (statue of liberty)." This poster represents our pledge and commitment to continuous allyship, with original image c/o Sammy Katta (https://sammykatta.com/diversity).
New preprint uploaded on bioRxiv from collaborators. Boysen et al., describe day-night cycles in microbial metabolites in the open ocean. Daily oscillations in cellular redox and energetics exert a strong influence on the surface ocean metabolome. Very exciting collaborative work with colleagues in the Simon's Foundation SCOPE Program. Check out the preprint here!
Image of R/V Kilo Moana during the diel study; c/o Tara Clemente.
Bruker's timsTOF fleX mass spectrometry system has arrived on UF's campus. The instrument will be operated in ICBR's mass spectrometry core. We are anxiously awaiting instrument setup and training once campus - and our lab - reopen.
Image of wooden boxes in UF's ICBR; c/o Sixue Chen.
Durham & former PhD advisor, Mary Ann Moran at the University of Georgia, have published a review article in Nature Reviews Microbiology entitled "Sulfur metabolites in the pelagic ocean." The co-authors examine how phytoplankton and bacteria produce & transform sulfur metabolites in the ocean, highlighting the critical role that sulfur plays in mediating microbial interactions and ocean ecosystem function. Link in image.
Cartoon representation of sulfur metabolites in the ocean that depicts some of their functions and the microbes that transform them.
Durham & colleagues from the University of Washington have published an article in Nature Microbiology, showing the importance of sulfonates in marine microbial interactions. Check out the blog post (link in image) and the manuscript here!
Image of sunset off the back deck of the R/V Kilo Moana.
Durham Lab | Department of Biology | University of Florida | Gainesville, FL